Stage-specific RNA-sequencing to identify genes differentially expressed between Toxoplasma gondii tachyzoites and bradyzoites. Stage-specific RNA-sequencing to identify genes differentially expressed between Toxoplasma gondii tachyzoites and bradyzoites

Using a differentiation reporter strain, unstressed parasites (tachyzoites) or stressed, reporter positive parasites (bradyzoites) were isolated by flow cytometry and analyzed for differential gene expression. Overall design: Data from three biological replicates. When possible, duplicate samples for each biological replicate and timepoint were taken, libraries prepared in parallel, and treated as technical replicates in downstream analysis