Analysis of CK1 inhibition effects on immune system in healthy and CLL-bearing mice

Casein kinase 1 (CK1) has been proved as an efficient approach in the treatment of chronic lymphocytic leukemia (CLL), as this therapeutic strategy led to longer overall survival and scarcer relapse in Eµ-TCL1 adoptive transfer (AT) mouse model. In this study, we aimed to find out whether the CK1 inhibition modulates the microenvironment of CLL in vivo and in what favor. Thus, we conducted several in vivo experiments on both healthy and CLL-bearing mice that were all terminated by multiparametric flow cytometric analysis of selected immune cell populations in 4 different types of microenvironment, including peripheral blood (PB), lymph nodes (LN), spleen (SPL) and bone marrow (BM).