H3K27ac ChIP-seq for multiple myeloma cell lines, B-lymphoma cells and normal plasma cell
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https://www.ncbi.nlm.nih.gov/sra/SRP250673
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资源简介：
ChIP-seq analysis was performed in Multiple Myeloma cell lines to analyze acetylation of histone H3K27ac. Overall design: Daudi, RAJI, H929, JJN3, KMS11, KMS12, KMS28BM, RPMI8226, U266 and NP (normal plasma cell) were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
创建时间：
2021-02-18
