Poly-A transcriptome changes in mouse embryonic fibroblasts (MEFs) after spontaneous immortalization

In our study, we characterized the changes in poly-A gene expression atributed to the process of spontaneous immortalization using a mouse embryonic fibroblasts (MEF) model. Primary MEFs were established, serially passaged and spontaneously immortalized using a modified 3T3 NIH protocol. We then performed gene expression profiling analysis using RNA-seq data of 3 different clones at two time points (primary and immortalized). Overall design: Gene expression profiling of RNA-seq data for MEF spontaneous immortalization.