Oxygen uptake in liver mitochondria isolated from euthyroid, hypothyroid and hypothyroid plus T2-treated rats.

Glutamate(5 mM)+malate (2.5 mM), succinate (5 mM) or a mixture of glutamate+malate+succinate (5, 2.5, 5 mM) were used as respiratory substrates. Oxygen consumption was measured by a Clark oxygen electrode. Respiratory activity of state 3, state 4 and FCCP-stimulated was expressed as natoms oxygen/min/mg of mitochondrial proteins. Respiratory control index (RCI) represents the ratio between state 3 and state 4 respiration rate. Data are means ± SD of 6 experiments performed with duplicate samples. Values sharing a different letter differ significantly. P