Data for: Walnut PR10/Bet v1-like proteins interact with chitinase in response to anthracnose stress

Walnut is a significant woody oil tree species that has been increasingly affected by anthracnose in recent years. Effective anthracnose control is crucial for walnut yield and quality, which requires a comprehensive understanding of the response mechanisms to Colletotrichum gloeosporioides. The PR10/Bet v1-like proteins are involved in defense against various diseases, therefore, in this study, 7 JrBet v1s were identified from the walnut transcriptome (named JrBet v1-1~1-7), whose open reading frame (ORF) was 414~483 bp in length with isoelectric point ranging from 4.96 to 6.11. These JrBet v1s were classified into three groups, with the MLP/RRP and Dicot PR-10 subfamilies each comprising three members (the largest ones), indicating that the proteins within these two subfamilies may have evolved from a shared ancestral gene within the broader PR10/Bet v1 protein family. The cis-elements in the promoters of JrBet v1s were involved in response to hormones, coercive, and plant growth meta..., 1 plant materials and treatments
Two-year-old ‘Xiangling’ walnut grafted seedlings were potted and used as materials (the growth temperature was 22±2℃, relative humidity was 70±5%, light cycle 14 h light/10 h dark). The C. gloeosporioides was isolated by the Laboratory of Walnut Research Center in Northwest A&F University, and cultivated to the conidia concentration of 105~106 cells/mL. Then spray the C. gloeosporioides spore re-suspension on the leaves for treatment after slight friction. The leaves were harvested after being treated with 1, 3, 6, 9, 12, and 15 days, and saved in a -80℃ refrigerator for further RNA isolation. Each treatment contained 6 plants.
2 Identification, collinearity, and structure analysis of JrBet v1s
“Pathogenesis-related protein Bet v1” was used to search for walnut Bet v1 family genes (JrBet v1s) in walnut transcriptome. The ORF Finder (https://www.ncbi.nlm.nih.gov/orffinder/) was used to find the open reading frame (ORF) of JrBet v1s. Basic biological ..., , # Data for: Walnut PR10/Bet v1-like proteins interact with chitinase in response to anthracnose stress

\[Access this dataset on Dryad](DOI: 10.5061/dryad.crjdfn3f8)

We have submitted the original datasets corresponding to all figures and tables presented in the manuscript. Specifically, the folders for Figures 1, 2, 3, 4, 5, S1, S2, S3, S4, and S5 contain the raw data used in the creation of these figures. Similarly, the folder for Table 1 includes the raw data utilized in the generation of Table 1.

## Description of the data and file structure

### **Figure 1**

#### The folder Figure 1 contains the following raw data:

* 7 walnut PR10-Bet v1 genes full length sequences.txt
* 7 walnut PR10-Bet v1 genes ORF sequences.txt
* 7 walnut PR10-Bet v1 genes full length sequence.txt

#### The methods of Figure 1

The Gene Structure Display Server 2.0 (GSDS 2.0; [http://gsds.gao-lab.org/](http://gsds.gao-lab.org/)) was utilized to construct the gene structure map (Hu et al., 2015). The open re...