Genome-Wide Profiling of Peroxisome Proliferator-Activated Receptor ? in Primary Epididymal, Inguinal, and Brown Adipocytes Reveals Depot-Selective Binding Correlated with Gene Expression

Here we have employed chromatin immunoprecipitation combined with deep sequencing to map and compare PPAR? binding in in vitro differentiated primary mouse adipocytes isolated from epididymal, inguinal, and brown adipose tissues. While these PPAR? binding profiles are overall similar, there are clear depot-selective binding sites. Most PPAR? binding sites previously mapped in 3T3-L1 adipocytes can also be detected in primary adipocytes, but there are a large number of PPAR? binding sites that are specific to the primary cells, and these tend to be located in closed chromatin regions in 3T3-L1 adipocytes. The depot-selective binding of PPAR? is associated with highly depot-specific gene expression. This indicates that PPAR? plays a role in the induction of genes characteristic of different adipocyte lineages and that preadipocytes from different depots are differentially preprogrammed to permit PPAR? lineage-specific recruitment even when differentiated in vitro. Overall design: Examination of PPAR? binding in in vitro differentiatied adipocytes isolated from three different adipose depots.