Data from: Avian offspring prenatal DNA methylation response to maternal corticosterone dosing during reproduction
收藏Mendeley Data2024-04-13 更新2024-06-27 收录
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https://datadryad.org/stash/dataset/doi:10.5061/dryad.kprr4xhbk
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# Avian offspring prenatal DNA methylation response to maternal corticosterone dosing during reproduction ## Description of the data files 1\) Embryo (5 days of incubation) DNA methylation assessed via LC-MS * Ratio of 5mdC (methylated cytosine) to guanosine for embryos (incubated to 5 days development) 2\) Egg (vitelline) blood vessel (from eggs with 5 days of incubation) DNA methylation assessed via LC-MS * Ratio of 5mdC (methylated cytosine) to guanosine for egg (vitelline) blood vessels (from eggs incubated to 5 days development) Both data sets (1 & 2) also include the following metrics: * Treatment group of mother (treatment: mothers remotely orally dosed with CORT daily; control: mothers remotely dosed with the oil vehicle, only, daily) * Mass in (g) of: mother finches (pre- and post- reproduction) and whole eggs (on day of lay, pre-incubation) * Length in (mm) of maternal tarsi * Clutch number (1st or 2nd); egg number (order laid in each clutch); and, clutch size (number of eggs laid in each clutch) * Dates (in dd/mm/yyyy format) for: nest material provided; nest building initiated; clutch initiated; first egg in clutch laid; and date focal egg laid * Lay latency (in whole days): between material provided date and first egg laid; and, between nest build initiation date and first egg laid 3\) Egg (vitelline) blood vessel (from eggs with 5 days of incubation) DNA methylation assessed via MS-AFLP * Absence/ presence for: Allele number (columns G-GH); DNA fragment length (columns GI-NJ); and peak height (columns NK-UL). * Restriction enzyme used to assess DNA methylation (HpaII or MspI) * Treatment group of mother (treatment: mothers remotely orally dosed with CORT daily; control: mothers remotely dosed with the oil vehicle, only, daily) * Maternal ID (also a proxy for parental pair ID, or familial group, as females were paired with the same male throughout all reproduction) * Clutch number (first or second clutch for each pair) * Egg number (order in the clutch each egg was laid)
创建时间:
2024-01-18



