RNA-seq revealed counter effect of chitosan against Botrytis cinerea on grape berries

Plants have great potential to protect against pathogens and other abiotic stress, and thus the interaction between signaling networks of defense is incredible. This can be activated and express with the application of growth elicitors and antimicrobial natural compounds. In the present study we used natural polysaccharide, Chitosan (15kDa) against Grey mold in two grape varieties (Shine-muscat and Kyoho). The finding depicted that the interaction of DEG and KEGG in control and treated samples of grapevine, which provides the evidence for selection of gray mold related defense genes and chitosan for subsequent grapevine production/postharvest programs. Overall design: Two Grape verities (Shine-muscat and Kyoho) treated with resistance inducers chitosan, the physiological changes in the grape berry were analyzed at 80-90 % physiologically maturities, with commercial grade chitosan, formulations were applied by foliar spray: 15% (w/v) chitosan (15 kDa) (Fig.1). To prepare formulations, we used distilled water as control. Each berry of grapy is treated as one replication and each replication is repeated together and randomized 3 times. One day later, Botrytis cinerea spores with 104 /µl concentration was used. The gray mold fungus was grown on grape berries at 25 °C, and 95 % relative humidity.