Chromatin interactions of the EVI1 oncogene in myeloid leukaemia (ATAC-Seq)

EVI1 expression is associated with poor prognosis in myeloid leukaemia. Aberrant expression can result from Chr.3q alterations, which cause juxtaposition of enhancers that induce EVI1 activation via long-range chromatin interactions. More often, however, EVI1 expression occurs unrelated to 3q alterations, and it is unclear if its expression underlies similar dependencies as 3q+ cells. As enhancers regulate promoters by physical interaction/chromatin looping, we explored if the EVI1 promoter in EVI1+3q- cells interacts with distally located chromatin and if these intereactions promote EVI1 expression. To monitor TF binding sites at interactions involving active chromatin, we performed ATAC-Seq. ATAC-Seq was performed on CML/AML cell lines according to the Omni-ATAC-seq protocol (https://doi.org/10.1038/protex.2017.096).