Effect of phytostimulatory seed-inoculant Azospirillum lipoferum CRT1 on total bacterial comminity (rrs) in the maize rhizosphere

The field trials were run in 2015 in Chatonnay (site P), Cessieu (site BV) and Saint Savin (site FV), which are located in the vicinity of Bourgoin-Jallieu (Isère, France). Maize seeds were inoculated or not by a suspension of Azospirillum lipoferum CRT1 cells. DNA was extracted from root-adhering soil and nifH gene diversity was sequenced using 515/806 targeting V4 variable region by Illumina Miseq (2 × 125 bp). Samples 261 to 265 correspond to site BV non-inoculated (NI) formulation F1. Samples 286 to 290 correspond to BV inoculated (I) formulation F1. Samples 311 to 315 correspond to FV-NI-F1. Samples 331 to 335 correspond to FV-I-F1 Samples 351 to 355 correspond to P-NI-F1. Samples 371 to 375 correspond to P-I-F1. Samples 271 to 275 correspond to site BV non-inoculated (NI) formulation F2. Samples 296 to 300 correspond to BV inoculated (I) formulation F2. Samples 321 to 325 correspond to FV-NI-F2. Samples 341 to 345 correspond to FV-I-F2 Samples 361 to 365 correspond to P-NI-F2. Samples 381 to 385 correspond to P-I-F2.