The effects of SOX4 on the global epigenetic landscape in Mammary Epithelial Cells

By chromatine-immunoprecipitating (ChIP) using the mammary epithelial cell line HMLE with a Doxycyclin-inducible overexpression of SOX4 we determined various histone modifications and presence of the POL2 polymerase in presence and absence of overexpressed SOX4. Overall design: ChIP with antibodies against trimethylated H3K4, acetylated H3K27, methylated H3K27 and Pol2 was performed in the ER-SOX4 HMLE cell line with and without doxycycline