Synergistic fusion of CD47, VE-cadherin and mussel adhesion protein promotes endothelialization and suppresses inflammation in vascular stents

Endothelial cell (EC)-specific coatings for vascular stents are crucial for enhancing their biocompatibility and preventing complications such as restenosis and thrombosis. This study developed an innovative CD47-VE-cadherin-Mfp5 (CD47-VE-M) fusion protein coating for cardiovascular stents that integrates three distinct functional domains: endothelial adhesion enhancement (VE-cadherin EC1-2), macrophage inhibitory signaling (CD47), and substrate adhesion reinforcement (Mfp5).In vitro, CD47-VE-M coatings significantly promoted EC adhesion (3.4-fold increase vs. bare-metal stent (BMS) (p in vivo. Besides, the coating also decreased the presence of M1 pro-inflammatory macrophages (64.74 % decrease vs. BMS (p Synergistic integration of CD47, VE-Cadherin, and mussel adhesion protein enhances endothelialization and attenuates inflammatory responses in vascular stents.Image 1View The PDF