Absolute copy number of mature miR-509 and miR-18a RNA per NALM6 cell.

RNA was isolated from NALM6 cells on day 7 after transduction with either control empty vector #1 or miR-509, and absolute qRT-PCR quantification was performed for miR-509-5p, miR-509-3p or miR-18a. Copy number per cell was estimated based on standard curves of miR-509-5p, miR-509-3p or miR-18a using DNA oligonucleotides. For reverse transcription, 10 ng RNA (equivalent to 800 cells, i.e. 12.5 pg of total RNA per cell) was used in each reaction. Means ± SEMs of 3 independent experiments.Absolute copy number of mature miR-509 and miR-18a RNA per NALM6 cell.