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Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases [I]

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NIAID Data Ecosystem2026-03-13 收录
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We adapted the iCLIP method to map ALKBH1L modification sites in a transcriptome-wide manner by treating the cells with 5-EC. Overall design: Flp-In T-Rex 293 cells expressing 3xFlag-ALKBH1 were treated with 5-EC, ALKBH1-RNA were extracted and subject for Iclip library preparation.

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2022-08-03
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