Supplementary data of Flower Pigments and the Color Expressions of Primula kisoana var. shikokiana (Primulaceae) and Its Breeding Lines

Table S1. Preliminar flower color evaluation of Primula kisoana var. shikokiana in 2017.*These data were measured from March to April 2017 (n=3).Table S2. Preliminar flower color evaluation of Primula kisoana var. shikokiana in 2023.*These data were measuredfrom March to April 2023 (n=3).Table S3. Absorption maxima of the intact petals of Primula kisoana var. shikokiana.*These data were measured from the end of March to April of 2017 and 2023.Table S4. UV−visible absorption spectra and LC/MS data of the isolated compounds.Table S5. The data of pH values in the petal juice of Primula kisoana var. shikokiana.*These data were measured from March to April 2017 (n=3).Fig. S1. The visible absorption spectra from the flowers of Primula kisoana var. shikokiana. The breeding lines are 92-1 (A, Orange group), 96C046 (B, Red group), PK-1b-4 (C, Red-Purple group), 04C083-2 (D, Purple group), 13C013-04 (E, Purple Violet group), 11C006-3 (F, Violet group), and 90-13 (G, Violet Blue group).Fig. S2. Relationships between flower colors and pH from the flowers of Primula kisoana var. shikokiana. Significant difference based on Tukey-Kramer’s multiple range test (P Fig. S3. Absorption spectra of in vitro reconstructed solutions. A = Anthocyanin solutions, 2 mM A2, A3, and A5 (solid line), and anthocyanin solutions containing 0.4 mM F2 (dashed line) at pH 5.0. B = The mixture solutions of A3+F2 at pH 5.5. The mixture proportion are 1 : 0 (a), 1 : 0.2 (b), 1 : 1 (c), and 1 : 3 (d).