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Determination of Z-factor and % CV for TSA in 384-well format.

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C2C12utrn cells cultured in 384-well plates were exposed to TSA at a range of concentrations. Peak luciferase activity occurred at 20 nM. To determine statistical robustness in 384-well format, the Z-factor and percentage covariance (% CV) were calculated at 5 and 20 nM. Z-factors between 0.5 and 1 predict good performance in high-throughput screening.

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2011-10-20
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