Evidence for intraspecific variation of salinity tolerance in zooplankton collected from lakes of differing salinities on the Great Plains

The Great Plains are expected to become drier over the coming decades as droughts caused by natural variation in precipitation are exacerbated by climate change. The resulting increases in lake salinity may impact biodiversity, including zooplankton. To better understand how salinity changes will affect zooplankton, our study evaluated intraspecific differences in salinity tolerance of zooplankton originating from lakes with differing salinity levels. Three species, including Ceriodaphnia laticaudata, Daphnia magna and Daphnia schodleri were collected from lakes near Saskatoon, Saskatchewan and were subjected to acute salinity tolerance tests. The 24-h and 48-h half-effective concentration (EC50) results for all three species were estimated using three replicate cultures established for each species. Calculations for EC50 values were conducted using the drc library in the R programming language. Comparisons of the EC50 values for individual species among lakes were made using ANOVAs. Th..., , # Evidence for intraspecific variation of salinity tolerance in zooplankton collected from lakes of differing salinities on the Great Plains

Dataset DOI: [10.5061/dryad.j0zpc86vv](https://doi.org/10.5061/dryad.j0zpc86vv)

## Description of the data and file structure

Experiments were conducted with three species of zooplankton collected from lakes near Saskatoon Saskatchewan to estimate salinity tolerance. EC50 values were calculated from the dose-response relationships using the drc package in the R programming environment.

### Files and variables

#### File: Appendix_analysis_code.Rmd

**Description:** This code reproduces the analysis in the associated publication, including calculating EC50 values and making figures. The code is in R Markdown format and can be opened with RStudio.

#### File: Supplementary_material_raw_data.csv

**Description:** This contains the data collected from salinity tolerance experiments used to construct dose response relationships between salinity and ...,