Biphasic Cell Cycle Defect Causes Impaired Neurogenesis in Down Syndrome [CPC RNA-Seq]

Using integrative approach involving human and mouse iPSCs, we have unravelded the cellular and molecular mechanisms of reduced neurogenesis in Down syndome Overall design: Isogenic normal and Down syndrome iPSCs were differentiated in to cortical progenitor cells (CPCs) for 24 day in vitro(DIV24). CPCs were used for total RNA isolation. A total of 6 samples that is 3 replicates of isogenic normal CPCs and 3 replicates of Down syndrome CPCs were used for mRNA-seq library preperation. Libraries wer subjected to single end high throughout sequencing.