Streptomyces ambofaciens time resolved transcriptome. Streptomyces ambofaciens ATCC 23877

In this study we have applied an integrated system biology approach to characterize the metabolic landscape of Streptomyces ambofaciens and to identify a list of potential metabolic engineering targets for the overproduction of the secondary metabolites in this microorganism. We focused on an often overlooked growth period (i.e. post-exponential phase) and, by integrating constraint-based metabolic modelling with time resolved RNA-seq data we depicted the main effects of changes in gene expression on the overall metabolic reprogramming occurring in S. ambofaciens. Moreover, through metabolic modelling, we identified a set of candidate overexpression gene targets hypothetically leading to spiramycin over-production.