Single-cell transcriptomic analysis of the scrib mutant wing imaginal discs

Investigation of intratumor heterogeneity in the scrib¹ mutant wing imaginal discs. Method: Staged scrib¹ wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina platform were performed by Novogene. 10X based single-cell mRNA profiles of 4, 5, 8, 14-day scrib mutant wing imaginal discs from prolonged larval stages.