Single-cell sequencing of human intestnal organoids with and without enteroendocrine cells (EECs)

We generated pluripotent stem cell-derived human intestinal organoids with (n=2) and without (n=1) EECs and performed single-cell sequencing after their transplantation under the mouse kidney capsule to evaluate similarities and differences in the absence of enteroendocrine cells. Overall design: Transplanted human intestinal organoids were dissociated to single cells, sorted by flow cytometry to enrich for live cells, then subjected to 10X sequencing.