Differential RNA expression in human neck and squamous cell carcinoma cell lines (T-HEp3 and D-HEp3).

We report the RNA expression profile in the tumorigenic (T-HEp3) and dormant (D-HEp3) HNSCC cell lines. T-HEp3 is a human epidermoid carcinoma, which grows on chorioallantoic membreane and metastasizes with high efficency into the organs of chick embryo. Upon serial paasage for more than 50 times in vitro , T-HEp3 loses tumorigenic and metastatic potential and termed as D-HEp3. This is an ideal cell line model system to study cancer dormancy. Overall design: RNA sequencing was performed in T-HEp3 (P0, primary cells extracted from tumor grown on chicken CAM and plated on dish) and D-HEp3 grown in vitro (passage later than 130).