Sample sizes for the biological assays used to collect data on the three DiGeorge subjects.

“# cells/RT reaction” is the number of cells used
in RNA extraction for spectratyping. The numbers were estimated from
the starting number of CD3+ cells and RNA yield.
All spectratyping used 150 ng of RNA regardless of the cellular
sample size. “CD4+ T cell
events” refers to the total number of flow cytometric
events in the CD3+CD4+ gate
used to determine the proportion of cells in each of 22 TCRBV
families as described in the text.