Sample sizes for the biological assays used to collect data on the three DiGeorge subjects.
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“# cells/RT reaction” is the number of cells used in RNA extraction for spectratyping. The numbers were estimated from the starting number of CD3+ cells and RNA yield. All spectratyping used 150 ng of RNA regardless of the cellular sample size. “CD4+ T cell events” refers to the total number of flow cytometric events in the CD3+CD4+ gate used to determine the proportion of cells in each of 22 TCRBV families as described in the text.
创建时间:
2009-06-12



