Taxus cuspidata Iso-Seq analysis

This project is to estimate the full-length amino acid sequences of Taxus cuspidata proteins with high reliability using the full-length cDNA sequences obtained by an isoform sequencing (Iso-Seq) analysis. RNA was extracted from the leaves on the shade side of a male Taxus cuspidata tree (height: around 8 m, age: 50 years old) grown in front of the Institute of Low Temperature Science, Hokkaido University (43.1°N, 141.3°E) at around 10 am on Jan 17 and Aug 8 in 2018. Poly A+ RNA was further purified and analyzed with Sequel II (Pacific Biosciences). The raw data was processed using SMRT Link v6.0 (Pacific Biosciences) and then further processed by IsoSeq3 tools (version 3.1).