Bulk RNA-seq of mouse heart tissue for analyzing gene expression of myocardial infarction progression

Myocardial infarction (MI) is a leading cause of heart failure (HF), which is associated with morbidity and mortality worldwide. Understanding the molecular characteristics of of MI is of significance for treating post-MI HF. In this study, RNA-seq was performed on heart tissue from mouse models at multiple time points (sham, 7d and 28d) to explore genetic features that influence MI progression. Overall design: Nine 8-week-old male C57BL/6JR mice were included in the experiment. The experiment was divided into 3 groups. Each group contained 3 mice, 2 groups of which required surgery to make models, called the operation group, and the other group was the sham operation group. The operation group was divided into 7d and 28d subgroups after MI. The mice were anaesthetized with isoflurane (1%-2%) and placed on the operating table. After anesthetizing mouse and left thoracotomy, the heart was exposed and the left anterior descending coronary artery was ligated with a 6-0 silk suture. The suture was passed 2-3 mm below the tip of the left auricle. And the sham group was subjected to the same procedures without LAD ligation.