ITC analysis of HTCS periplasmic domains binding to oligo- and polysaccharides.

aLocus tag of HTCS with polysaccharide that activates associated PUL in parentheses.bNB, no binding.cData are averages and SDs of at least triplicate titrations at 25°C, 50 mM Hepes buffer, pH 8.0. Binding of oligosaccharides to BT0366 and both oligo- and polysaccharides to BACOVA_02097 were fit to a two-site binding model. Values reported are for site 1 then site 2.dRepresentative titration curves are provided in Figure S7.eFit as ligand in cell.fTLTF, too low to fit (i.e., binding event observed, but affinity too low to accurately fit the data. Ka2 M−1).gMegazyme cat. no. OGGM5.Each HTCS sensor domain was also evaluated for binding to the other oligosaccharides tested from non-inducing glycan sources. In all cases, no binding was detected.