Expression data from rat skeletal muscle

We extracted skeletal muscle RNA from a total of 48 female animals (n=6 in each of the 8 group). Skeletal muscle tissue was obtained from theExtensor digitorum longus(EDL). All rats were dissected immediately after sacrificing, and all tissue samples were immediately weighed and snap frozen in liquid nitrogen, and stored at -80 ºC. Total RNA was extracted from frozen tissue with Trizol reagent (Invitrogen, Carlsbad, CA), treated with DNase-free (Invitrogen, Carlsbad, CA) and cleaned up with RNeasy columns (Qiagen, Hilden, Germany)本研究从共计48只雌性实验动物中提取骨骼肌RNA，分为8个组别，每组n=6。骨骼肌组织取材于趾长伸肌（Extensor digitorum longus，EDL）。所有大鼠处死后立即实施解剖，所有组织样本均即刻称重后经液氮快速冷冻，并保存于-80℃环境中。采用Trizol试剂（Invitrogen，美国加利福尼亚州卡尔斯巴德市）从冷冻组织中提取总RNA，经DNase-free试剂（Invitrogen，美国加利福尼亚州卡尔斯巴德市）处理后，通过RNeasy柱（Qiagen，德国希尔德斯海姆市）完成纯化。