A link between mitotic entry and membrane growth suggests a novel model for cell size control

The pkc1-14 mutant compromises functions of Pkc1 that are important when growth is slowed due to nutrient limitation, and the effects of the pkc1-14 mutant can be rescued by loss of Cdk1 inhibitory phosphorylation in a swe1Δ mutant background. The control (wild-type) yeast cells shown here exhibit no visible bud elongation. These cells were inoculated into YPD media at low density and grown at 30°C until they reached an optical density of approximately 1.7. Cells were fixed with formaldehyde and photographed using a Zeiss Axioskop microscope fitted with a 100X Plan-NEOFLUAR 1.3 objective and a Axiocam HRm camera. Images were acquired using Zeiss Axiovision software.