Effect of growth in TSA or SCFA on gene expression in E. histolytica

In order to dtermine the role of histone actylation in gene expresssion, E. histolytica 200:NIH trophozoites were treated with the HDAC inhibitor Trichostatin A, or grown in short chain fatty acids, which have been shown to alter histone actylation patterns in Entamoeba species. Keywords: Expression E. histolytica 200:NIH trophozoites grown in standard media (TYI or TYI-low glucose) were compared to trophozoites grown in TYI-LG + Trichostatin A (150 or 300 nM) for 16 or 72 hours, or in TYI-LG + short chain fatty acids (70 mM sodium acetate, 20 mM sodium propionate and 10 mM sodium butyrate). Differences in gene expression were assayed by hybridization to a full genome Affymetrix array.