Clostridium acetobutylicum ATCC 824 (pLib1) butanol stresses with repeated transfers

Metabolite accumulation has pleiotropic, including toxic, effects on cellular physiology, with a variety of genetic resistance mechanisms previously identified. Using random genomic libraries and DNA microarrays, library inserts were preferentially enriched by culturing in media containing butanol, with successive transfers into fresh media containing incrementally increasing butanol concentrations. Keywords: genomic library preferential enrichment plasmid DNA samples were taken from individual transfers (4th, 7th, 10th, and 13th transfers) of butanol challenged cultures and hybridized against plasmid DNA isolated from the culture used to inoculate the first challenge culture. Two biological replicate challenge experiments were performed and respective microarrays hybridized.