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CUT&Tag assay was performed according to the previous protocols (PMID: 31036827). The size distribution of libraries was determined by automated electrophoresis (Agilent), and libraries were mixed to achieve equal representation as desired. DNA sequencing was performed by the Novaseq 6000 System (Illumina) using a 150bp paired-end following the manufacturer’s instructions.

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2023-12-18
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