Impact of HSF1 and HSF2 on gene expression in normal growth conditions and upon proteotoxic stress.. Impact of HSF1 and HSF2 on gene expression in normal growth conditions and upon proteotoxic stress.

RNA sequencing (RNA-seq) was used to study the impact of heat shock factors 1 and 2 (HSF1 and HSF2) on gene expression in normal growth conditions, upon Bortezomib-induced proteasome inhibition (25 nM, 6 h or 10 h), and upon heat shock (42∘C, 1 h). Overall design: U2OS WT, HSF1 KO, and HSF2 KO were treated with 25 nM of Bortezomib for 6 h or 10 h, or heat shocked at 42 degrees for 1 h. Samples were prepared in biological quadruplets.