The complete chloroplast genome and phylogenetic analysis of <i>Rubus sumatranus</i> Miq 1861 (Roseaceae)
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The wild raspberry species <i>Rubus sumatranus</i> Miq 1861 is a promising resource for breeding thermotolerant cultivars. Its complete chloroplast genome spans 155,935 base pairs (bp), featuring the classic quadripartite structure: an 18,729 bp small single-copy region, an 85,662 bp large single-copy region, and two 25,772 bp inverted repeats. A total of 130 genes were identified, including 86 protein-coding, 36 tRNA genes, and 8 rRNA genes. A maximum likelihood phylogenetic tree based on chloroplast genomes shows that <i>R. sumatranus</i>, within the subgenus <i>Idaeobatus</i>, is sister to the subgenus <i>Batothamnus</i>. This confirms the polyphyletic nature of the subgenus <i>Idaeobatus</i>. The chloroplast genome assembly of <i>R. sumatranus</i> enhances our understanding of its evolutionary history.
野生悬钩子属物种苏门答腊悬钩子(*Rubus sumatranus* Miq 1861)是培育耐热栽培品种的极具潜力的种质资源。其完整叶绿体基因组全长155935个碱基对(bp),具有典型的四分体结构:包含18729 bp的小单拷贝区、85662 bp的大单拷贝区,以及两段各长25772 bp的反向重复序列。本研究共鉴定出130个基因,其中包括86个蛋白编码基因、36个转运RNA(transfer RNA, tRNA)基因以及8个核糖体RNA(ribosomal RNA, rRNA)基因。基于叶绿体基因组构建的最大似然系统发育树显示,隶属于树莓亚属(*Idaeobatus*)的苏门答腊悬钩子与巴托莓亚属(*Batothamnus*)互为姊妹群,这一结果证实了树莓亚属具有多系起源的特性。苏门答腊悬钩子的叶绿体基因组组装结果,有助于深化我们对其进化历史的认知。




