Transcription analysis of genes involved in sucrose metabolism, acetoin, and 2,3-butanediol synthesis in <i>Enterobacter</i> sp. 638 when grown in the presence of sucrose or lactate as carbon source.

Cultures of Enterobacter sp. 638 were grown for either 6 or 8 hours in the presence 0.2% sucrose or lactate as sole carbon source, after which cells were harvested. Their total RNA was isolated, and after removal of 16S and 23S rRNA converted into cDNA and used for Q-PCR analysis. Expression of recA (encoding a protein involved in DNA repair) was used as an internal control to normalize gene expression. Results are given in fold induction of cells grown on sucrose as a sole carbon source with respect to the culture on lactate as a sole carbon source, and are mean ± SD (N = 3).