In vitro potency and physicochemical properties for bicyclic 4-nitroimidazole analogs.

MIC99 = Minimum Inhibitory Concentration required to reduce the bacterial growth by 99%, MIC against both H37Rv (wild type) and F420 deficient (FbiC) mutants were tested. PAMPA = Parallel Artificial Membrane Permeability Assay, CaCo-2 = Permeability using colon carcinoma cell lines, PPB = Plasma Protein Binding, Pe = effective permeability, FA = fraction absorbed, Papp = apparent permeability, A-B = Apical to Basolateral, B-A = Basolateral to Apical, nd = not determined, CC50 = Cytotoxicity against THP1 macrophage cell lines was determined as described previously [76], with Puromycin as positive control (CC50 = 1.4 mg/L).