Source data for Noncoding transcription controls the developmental dynamics of long-range gene regulation

This repository contains live microscopy data acquired in Drosophila melanogaster embryos for transcription measurements carried out using the MS2/PP7 system. The data is organized into directories corresponding to the genotype of the embryos imaged. Directory name Embryo Genotype AntpEE2PP7_AntpMS2_LSM900 AntpEE2-PP7 x Antp-MS2 AntpEE2PP7_AntpMS2ncRNAdelTSS_LSM900 AntpEE2-PP7 x Antp-MS2, ncRNADTSS eRNAMS2_ScrPP7_Cis Scr_eRNA-MS2, Scr-PP7 (Cis) eRNAPP7_ScrMS2_Trans Scr_eRNA-PP7 x Scr-MS2 (Trans) ScrEEPP7_dtRNA_MS2 ScrEE-PP7 x Scr_dtRNA-MS2 ScrEEPP7_dtRNAMS2_DELeRNAtss ScrEE-PP7 x Scr_dtRNA-MS2, eRNADTSS ScrEEPP7_dtRNAMS2_DELscrEE ScrEE-PP7 x Scr_dtRNA-MS2, DScrEE ScrEEPP7_eRNAdelTSSMS2 ScrEE-PP7 x Scr_eRNA-MS2, eRNADTSS ScrEEPP7_eRNAevePMS2 ScrEE-PP7 x Scr_eRNA-MS2, eveP>eRNA ScrEEPP7_eRNAMS2 ScrEE-PP7 x Scr_eRNA-MS2 ScrEEPP7_eRNAMS2delDTE ScrEE-PP7 x Scr_eRNA-MS2, DDTE ScrEEPP7_ScrEEMS2_Reporter ScrEE-PP7 x ScrEE-MS2 (Reporter) ScrEEPP7_ScrEEMS2delTSS_Reporter ScrEE-PP7 x ScrEE-MS2, DeRNA_TSS (Reporter) ScrEEPP7_ScrMS2 ScrEE-PP7 x Scr-MS2 ScrEEPP7_ScrMS2_LSM900 ScrEE-PP7 x Scr-MS2_LSM900 ScrEEPP7_ScrMS2delDTE ScrEE-PP7 x Scr-MS2, DDTE ScrEEPP7_ScrMS2dtRNAdelTSS ScrEE-PP7 x Scr-MS2, dtRNADTSS ScrEEPP7_ScrMS2eRNAdelTSS ScrEE-PP7 x Scr-MS2, eRNADTSS ScrEEPP7_ScrMS2eRNAdelTSS_delDTE ScrEE-PP7 x Scr-MS2, eRNADTSS, DDTE ScrEEPP7_ScrMS2eRNAdelTSS_LSM900 ScrEE-PP7 x Scr-MS2, eRNADTSS ScrEEPP7_ScrMS2eRNAeveP ScrEE-PP7 x Scr-MS2, eveP>eRNA ScrEEPP7_ScrMS2eRNAeveP_LSM900 ScrEE-PP7 x Scr-MS2, eveP>eRNA ScrEEPP7_ScrMS2eRNAevePmInr_LSM900 ScrEE-PP7 x Scr-MS2 evePmInr>eRNA ScrEEPP7_ScrMS2eRNAlambda_LSM900 ScrEE-PP7 x Scr-MS2, eRNAlambda ScrEEPP7_ScrMS2eRNAlambdaSV40pA_LSM900 ScrEE-PP7 x Scr-MS2, eRNAlambdaSV40pA ScrEEPP7_ScrMS2eRNAtss_Trans eRNADTSS x Scr-MS2 (Trans) There are 6 tab-separated text files derived from each 4D image dataset corresponding to a single embryo (biological replicate), which share the same name followed by a suffix: · _GFP_Signal.txt Raw transcription spot intensity in MS2 channel (MCP-GFP) · _GFP_Background.txt Diffuse nuclear signal in MS2 channel (MCP-GFP) · _RFP_Signal.txt Raw transcription spot intensity in PP7 channel (mCherry-PCP) · _RFP_Background.txt Diffuse nuclear signal in PP7 channel (mCherry-PCP) · _Nucleus_X.txt X coordinates of nucleus segmentation mask centroids · _Nucleus_Y.txt Y coordinates of nucleus segmentation mask centroids Rows correspond to individual nuclei (tracked over time). Columns correspond to time points (Z-stacks). Coordinates are in pixels. Signal/background are in fluorescence units. Directories with names ending in ‘LSM900’ contain data acquired on a Zeiss LSM900 Airyscan2 confocal microscope; all other directories contain data acquired on a Zeiss LSM880 Airyscan confocal microscope (see detailed methods in linked publication). The file named Batut2026_SexCombCounts.txt contains raw counts of sex comb teeth for adult male individuals of the following genotypes (corresponding to columns 1-4, in order): · ScrMS2 Scr-MS2 · ∆ScrDTE Scr-MS2, DDTE · ∆eRNA Scr-MS2, eRNADTSS · ∆dtRNA Scr-MS2, dtRNADTSS