DHX36 resolves G-rich structures in mRNA untranslated region to allow their translation [cPDS-RNA-seq]

DHX36 is a ATP-dependent, 3´-5´ RNA helicase of the DEAH family. Previous publications reported this helicase to associate with AU-rich elements and to specifically unwind G-quadruplex structures. Here, we performed RNA-seq in triplicats of HEK293 wildtype cells and DHX36-KO-HEK293 cells to identify changes in RNA abundance upon presence or absence of this helicase. After sequencing and mapping to the human genome at nucleotide-resolution level we analysed the mRNA abundance of target mRNAs, identified in this study (see PAR-CLIP). Our analyses show that binding of DHX36 to 3´UTRS of target mRNAs significantly reduce their abundance. Examination of RNA abundance in HEK293 wildtype cells and DHX36-KO-HEK293 cells upon treatment with RNA-G4 stabilizing ligand carboxyPyridostatin