3-way-aligned-orthologous-segments

This FASTA-formatted file is a series of 56 sequence alignments. Each alignment contains one sequence from each frog transcriptome studied. The sequences were aligned at the protein level using MUSCLE (Edgar 2004) and then converted to nucleotide alignments. Annotation information for each set of contigs can be found in the two annotation.xls spreadsheets. This file provides the expected amplicons from each reference sequence for the primers listed in conserved-primer-candidates-for-orthologous-segments.xls, for the purposes of guiding the selection of sequences that may be useful for a given population-genetic or phylogenetic study.