Reduced Representation Bisulfite Sequencing (RRBS) of fluorescence-activated cell sorted (FACS) human spermatozoa stained with Chromomycin A3 (CMA3) and YO-PRO-1 (YOPRO) in fertile donors and infertile patients with short anogenital distances.

Infertile patients with short AGD are considered as having poorly been exposed to androgen during their past fetal lives. Sub-populations of spermatozoa were selected for their protamine content (CMA3+ versus CMA3-) and for apoptosis (YOPRO+ versus YOPRO-). Pairwise comparisons of the epigenomic data between donors and patients, ranked according to the staining and sorting results: C3_[YOPRO-_patients]_VS_[YOPRO-_donors] C8_[YOPRO+patients]_VS_[YOPRO+_donors] C2_[CMA3-_patients]_VS_[CMA3-_donors] C9_[CMA3+_patients]_VS_[CMA3+_donors] Pairwise comparisons of the epigenomic data according to the staining and sorting results: C6_[YOPRO-_donors]_VS_[YOPRO+_donors] C7_[YOPRO-_patients]_VS_[YOPRO+_patients] C5_[CMA3-_donors]_VS_[CMA3+_donors] C4_[CMA3-_patients]_VS_[CMA3+_patients]