Genome-wide expression in asymmetric histone H3 mutants in Saccharomyces cerevisiae

Using histone H3 mutants designed so that they can only form a heterodimer, we studied the gene expression in a collection of mutants, comparing mutations to one histone H3 tail of a nucleosome compared to two histone H3 tails of a nucleosome. Overall design: RNA-Seq on 13 different histone H3 mutants present in asymmetric or symmetric form, as well as 8 additional histone H3 mutants present in asymmetric form (symmetric form was presumed lethal in these remaining).