Microarray comparing CMV-specific CD8+ T cells from liver sinusoid and peripheral blood. Microarray comparing CMV-specific CD8+ T cells from liver sinusoid and peripheral blood

The liver is an immunologically unique organ in terms of the regulation of immuno-tolerance and immune responses. Recently, non-recirculating tissue-resident memory T (TRM) cells expressing CD69 were found in various peripheral organs. In humans, a large population of CD69+CD8+ memory T cells residing in liver sinusoids shows reduced cytotoxicity. However, the trascriptional characteristics of human liver CD8+ T cells are still unknown. Therefore, we performed a microarray analysis to screen the gene expression profile in liver sinusoidal CD8+ T cells using CMV-specific CD8+ T cells from human peripheral blood and liver sinusoid. Overall design: We performed microarray analysis after sorting HLA-A*0201-CMVpp65495 pentamer+CD8+ T cells from paired LSMCs and PBMCs.