Raw data of manuscript named"Nanoparticles blocking fibroblasts-to-macrophages communication for chronic heart failure therapy"

Nanomaterials may be potential candidates for targeting intercellular interactions. However, whether nanomaterials affect intercellular interactions to improve the occurrence and development of diseases remains unknown. Here, we construct Prussian blue (PB) nanoparticles that can be mass-produced, but encounter an uncommon phenomenon in chronic heart failure (HF). PB nanoparticles are ineffective in single-cell system but have good therapeutic efficacy in different mouse models of HF. Single-nucleus RNA sequencings of mice and human samples, gene knockout mice, and molecular dynamics simulation unravel that PB nanoparticles directly binds to CCL2, blocking the intercellular communications between a subcluster of cardiac fibroblasts with both myofibroblasts properties and immune-modulating capacities to recruited CCR2+ macrophages. Through this mechanism, PB nanoparticles showed significant therapeutic efficacy and biosafety in a pig HF model. This study highlights insights into how nanomaterials affect intercellular interactions and demonstrates an unpredictable treatment of HF, proposing a paradigm for studying the intrinsic bioactivities of nanomedicines in microenvironmental homeostasis.