Sequencing of Lymniadeae from Estonia and Germany. Radix ITS

For internal transcribed spacer 2 (ITS2) analysis, the DNA were PCR amplified according to a protocol from Vinarski et al (2011) using the primers LT1 (Bargues et al. 2001) and ITS2-Rixo (Almeyda-Artigas et al. 2000). The temperature profile used was 94°C 4 min (94°C 30 s, 56°C 30 s, 72°C 1 min) × 35, 72◦C 7 min, 8°C. The amplicons were purified using PCR Kleen (Bio-Rad) and Sanger sequenced by the sequencing facility of the Tartu University, Estonia.