Transcriptome analysis of BACH2 LTR-edited human samples

This work focused on modeling the effects of HIV insertions into commonly targeted genes (BACH2) using CRISPR/CAS9 targeted gene editing. CD4+ T cells were isolated for donor PBMCs and edited with RNPs at intron 5 of BACH2 with a single HIV LTR element (Solo-LTR) or a LTR-MSD (major splice donor) cassette delivered by AAV. These cells were then cultured over extended periods during which phenotypic analysis was done by flow cytometry, and transcriptome analysis done by bulk RNAseq.