Targets for renal carcinoma growth control identified by screening FOXD1 cell proliferation pathways

FOXD1 regulates proliferation of clear cell renal cell carcinoma (ccRCC) cells, and ccRCC cells in which FOXD1 has been inactivated do not form tumors efficiently in an animal model. Reproducing growth inhibition in tumor cells by inhibiting FOXD1 pathways presents an possible therapeutic approach for ccRCC and other cancers. We have established an analysis strategy to identify FOXD1-regulated target pathways that may be therapeutically tractable and compounds that modulate these pathways were selected for testing. Biological triplicate total RNA extracts from cell lines 786-O and 786-OFOXD1null were paired-end sequenced.