RNA-seq data to map the AtsR regulon in B. cenocepacia. RNA-seq data to map the AtsR regulon in B. cenocepacia

In this study, we embark on a journey to unravel the profound impact of atsR (Adherence and T6SS Regulator) gene deletion on the transcriptome and the proteome of B. cenocepacia K56-2 upon growth in synthetic CF medium (SCFM), the most in-vivo-like culturing medium. Our insights, derived from RNA-seq analysis and label-free LC-MS-based proteomics, validated by multiple reaction monitoring (MRM) analysis, reveal that AtsR's influence extends beyond adhesion, T6SS and the two above QSs, encompassing valdiazen QS, phenylacetic acid signaling system, diffusible signaling factor production, and other pathogenesis-modulating elements such as alternative sigma factors, small regulatory RNAs, and several chaperones.