Ability of various nucleoside analogues to inhibit HEK293T-cell apoptosis induced by nodularin1.

1: HEK293 cells transfected with either OATP1B1 or OATP1B3 were incubated with increasing doses of cAMP analogs for 15 min before addition of Nod (0.1 µM for OATP1B1 and 0.6 µM for OATP1B3). After 90 min coincubation, the cells were fixed in 2% buffered formaldehyde, and percent apoptotic cells determined by microscopic evaluation of surface membrane. The data was used to estimate IC50 values using a four parameter curve fitting (see Experimental section). The panels show typical data for cAMP analogs inhibiting apoptosis (left) or analogs that do not interfere with apoptosis (right). All experiments were performed in triplicates or more. The R2 for each regression analysis is shown.