Transcriptome changes of endothelial cells in mouse breast tumors by STING activation II

In order to identify transciptomic changes of endothelial cells (Ecs) in response to STING activation, endothelial cells were soted using FACS and RNA-seq was performed. We compared ECs from 3 models; normal mammary fat pad, MMTV-PyMT spontaneous breast tumor, implanted breast tumor. Tumor cells derived from MMTV-PyMT spontaneous breast tumor were expanded on culture and implanted in mammary fat pad of female FVB mice to establish implanted breast tumor model. Overall design: PBS or cGAMP (STING agonsit) was directly injected to mammary fat pad or tumors. Samples were harvested 3 hr later.